BCIP p-toluidine salt
≥99%(HPLC)
- Product Code: 97982
Alias:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
CAS:
6578-06-9
| Molecular Weight: | 433.62 g./mol | Molecular Formula: | C₁₅H₁₅BrClN₂O₄P |
|---|---|---|---|
| EC Number: | 229-506-1 | MDL Number: | MFCD00040636 |
| Melting Point: | 194-195℃ | Boiling Point: | |
| Density: | Storage Condition: | -20 °C |
Product Description:
BCIP p-toluidine salt is widely used in molecular biology and biochemistry as a substrate for alkaline phosphatase in various detection assays. It is particularly valuable in techniques like Western blotting, ELISA, and immunohistochemistry, where it helps visualize proteins or nucleic acids. When cleaved by alkaline phosphatase, it produces an insoluble indigo dye, which forms a blue precipitate, allowing for precise localization of target molecules. This property makes it a crucial tool in research labs for studying protein expression, cellular localization, and diagnostic applications. Its sensitivity and reliability contribute to its widespread use in both academic and clinical settings.
Product Specification:
| Test | Specification |
|---|---|
| SULFATED ASH | 0-0.05 |
| O.D. 620NM 0.1 DMF | 0-0.5 |
| PURITYHPLC | 99-100 |
| WATER BY KARL FISCHER | 0-2 |
| APPEARANCE | WHITE TO LIGHT YELLOW-TAN POWDER |
| IDENTIFICATION | PASS |
| SOLUBILITY IN DMF | CLEAR SLIGHTLY HAZY YELLOW SOLUTION AT 20 MG/ML |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.100 | 10-20 days | $70.39 |
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| 0.500 | 10-20 days | $235.17 |
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| 1.000 | 10-20 days | $392.87 |
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| 5.000 | 10-20 days | $1,572.25 |
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BCIP p-toluidine salt
BCIP p-toluidine salt is widely used in molecular biology and biochemistry as a substrate for alkaline phosphatase in various detection assays. It is particularly valuable in techniques like Western blotting, ELISA, and immunohistochemistry, where it helps visualize proteins or nucleic acids. When cleaved by alkaline phosphatase, it produces an insoluble indigo dye, which forms a blue precipitate, allowing for precise localization of target molecules. This property makes it a crucial tool in research labs for studying protein expression, cellular localization, and diagnostic applications. Its sensitivity and reliability contribute to its widespread use in both academic and clinical settings.
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