3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate
98%
- Product Code: 126770
Alias:
TMB HCL ; 3,3',5,5'-tetramethylbenzidine dihydrochloride dihydrate
CAS:
64285-73-0
| Molecular Weight: | 313.27 g./mol | Molecular Formula: | C₁₆H₂₂Cl₂N₂ |
|---|---|---|---|
| EC Number: | 264-769-6 | MDL Number: | MFCD00012961 |
| Melting Point: | ≥300 °C | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
Used extensively as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) for detecting peroxidase activity. It produces a blue color upon oxidation, which turns yellow when acid is added, allowing for quantitative analysis. Commonly applied in clinical diagnostics for detecting antibodies, antigens, and hormones. Also utilized in immunohistochemistry for visualizing tissue samples and in research laboratories for studying enzyme kinetics. Its sensitivity and stability make it a preferred choice in various biochemical and medical applications.
Product Specification:
| Test | Specification |
|---|---|
| Purity (HPLC) (%) | 98 |
| Purity (Titration With AgNO3) (%) | 97.5-102.5 |
| Water By Karl Fischer (%) | 10-12 |
| Appearance | White Or Light Yellow Powder |
| Infrared Spectrum | Conforms To Structure |
| Solubility In Acetic Acid:Water (1:2) | Clear To Slightly Hazy Yellow To Green, At 20mg/mL |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.250 | 10-20 days | $19.93 |
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| 1.000 | 10-20 days | $41.11 |
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| 5.000 | 10-20 days | $162.38 |
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| 25.000 | 10-20 days | $662.80 |
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3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate
Used extensively as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISA) for detecting peroxidase activity. It produces a blue color upon oxidation, which turns yellow when acid is added, allowing for quantitative analysis. Commonly applied in clinical diagnostics for detecting antibodies, antigens, and hormones. Also utilized in immunohistochemistry for visualizing tissue samples and in research laboratories for studying enzyme kinetics. Its sensitivity and stability make it a preferred choice in various biochemical and medical applications.
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