Phenylmethanesulfonyl fluoride (PMSF)
≥98%(GC)
- Product Code: 123168
Alias:
phenylmethylsulfonyl fluoride (PMSF); phenylmethylsulfonyl fluoride
CAS:
329-98-6
| Molecular Weight: | 174.19 g./mol | Molecular Formula: | C₇H₇FO₂S |
|---|---|---|---|
| EC Number: | 206-350-2 | MDL Number: | MFCD00007424 |
| Melting Point: | 92-95 °C | Boiling Point: | 112 °C16 mm Hg(lit.) |
| Density: | 0.797 g/mL at 20 °C | Storage Condition: | 2~8°C, combustible area, sealed |
Product Description:
PMSF is widely used as a serine protease inhibitor in biochemical research. It is commonly added to cell lysis buffers to prevent protein degradation during the extraction process, ensuring the integrity of proteins for analysis. In molecular biology, PMSF is employed to inhibit proteases that may interfere with experiments involving DNA, RNA, or protein isolation. It is also utilized in the study of enzyme mechanisms, particularly for serine proteases like trypsin and chymotrypsin, to block their activity. Additionally, PMSF is applied in the preparation of samples for techniques such as Western blotting and chromatography to maintain protein stability. Its rapid action and effectiveness make it a valuable tool in laboratory settings, though it requires careful handling due to its toxicity.
Product Specification:
| Test | Specification |
|---|---|
| Melting Point | 91-95 |
| Purity (GC) | 98.5-100 |
| Appearance | White Or Light Yellow Powder |
| Proton NMR Spectrum | Conforms To Structure |
| Solubility In Isopropanol | Clear To Slightly Hazy At 0.2 M (139.2 MG Plus 4ML) |
| Solubility In Methanol | Almost Transparency |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 1.000 | 10-20 days | $17.40 |
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| 5.000 | 10-20 days | $35.40 |
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| 25.000 | 10-20 days | $118.51 |
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| 100.000 | 10-20 days | $443.74 |
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Phenylmethanesulfonyl fluoride (PMSF)
PMSF is widely used as a serine protease inhibitor in biochemical research. It is commonly added to cell lysis buffers to prevent protein degradation during the extraction process, ensuring the integrity of proteins for analysis. In molecular biology, PMSF is employed to inhibit proteases that may interfere with experiments involving DNA, RNA, or protein isolation. It is also utilized in the study of enzyme mechanisms, particularly for serine proteases like trypsin and chymotrypsin, to block their activity. Additionally, PMSF is applied in the preparation of samples for techniques such as Western blotting and chromatography to maintain protein stability. Its rapid action and effectiveness make it a valuable tool in laboratory settings, though it requires careful handling due to its toxicity.
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