Protein Size Measurement (8.5KDa - 100KDa, SDS-PAGE)

Protein Size Measurement (8.5KDa - 100KDa)

Service Steps

Step	Procedure	Expected Result
1	1...	Obtaining 10% Ammonium per Sulfate (APS) solution.
2	10...	Obtaining 10% Sodium dodecyl sulfate (SDS) solution.
3	100...	Obtaining1M Tris Hydrochloric Acid , pH 6.8.
4	100...	Obtaining1.5M Tris Hydrochloric Acid, pH 8.8.
5	100...	Obtaining 30% Acrylamide solution.
6	1000...	Obtaining 10X Laemmli electrode buffer solution.
7	1000...	Obtaining 1X Laemmli electrode buffer (working) solution.
8	10...	Obtaining 2X Sample loading buffer (with reducing agent) solution.
9	7...	Obtaining 10% of separating gel.
10	7...	Obtaining 12.5% separating gel.
11	10.205...	Obtaining 15% separating gel.
12	10.205...	Obtaining 18% separating gel .
13	10.21...	Obtaining 20% separating gel.
14	2.5...	Obtaining Stacking gel .
15	Dissolving...	Obtaining fixing solution .
16	Dissolving 0.25%...	Obtaining Staining Solution.
17	Dissolving 5%...	Obtaining Destaining Solution.
18	Reagent...	Requirement tools .
19	Prepare...	Reaction occurs fold protein to unfold protein .
20	Add...	Formation of a blue-colored complex between the test sample and reaction solution.
21	Choose...	To run the Molecular Size of Sample.
22	To run...	To run the Molecular Size of Sample.
23	The gel...	To fix color of gel layer before dying.
24	Stain the...	To dye the gel layer.
25	Destain...	Obtaining the clear gel layer.
26	Compare the...	Obtaining the approximate protein molecular weight in unknown sample.

You will receive a report for the The value of molecular weight in protein (kDA) fields when we provide this service

Protein Size Measurement (8.5KDa - 100KDa, SDS-PAGE)

Protein Size Measurement (8.5KDa - 100KDa)

Service Steps

Step	Procedure	Expected Result
1	1...	Obtaining 10% Ammonium per Sulfate (APS) solution.
2	10...	Obtaining 10% Sodium dodecyl sulfate (SDS) solution.
3	100...	Obtaining1M Tris Hydrochloric Acid , pH 6.8.
4	100...	Obtaining1.5M Tris Hydrochloric Acid, pH 8.8.
5	100...	Obtaining 30% Acrylamide solution.
6	1000...	Obtaining 10X Laemmli electrode buffer solution.
7	1000...	Obtaining 1X Laemmli electrode buffer (working) solution.
8	10...	Obtaining 2X Sample loading buffer (with reducing agent) solution.
9	7...	Obtaining 10% of separating gel.
10	7...	Obtaining 12.5% separating gel.
11	10.205...	Obtaining 15% separating gel.
12	10.205...	Obtaining 18% separating gel .
13	10.21...	Obtaining 20% separating gel.
14	2.5...	Obtaining Stacking gel .
15	Dissolving...	Obtaining fixing solution .
16	Dissolving 0.25%...	Obtaining Staining Solution.
17	Dissolving 5%...	Obtaining Destaining Solution.
18	Reagent...	Requirement tools .
19	Prepare...	Reaction occurs fold protein to unfold protein .
20	Add...	Formation of a blue-colored complex between the test sample and reaction solution.
21	Choose...	To run the Molecular Size of Sample.
22	To run...	To run the Molecular Size of Sample.
23	The gel...	To fix color of gel layer before dying.
24	Stain the...	To dye the gel layer.
25	Destain...	Obtaining the clear gel layer.
26	Compare the...	Obtaining the approximate protein molecular weight in unknown sample.

Cart

No products

Subtotal: ฿0.00

฿0.00 Total :