significantly reduces melanin production and visibly brightens skin, outperforming other agents like Kojic Acid and Arbutin, with superior safety and stability

ChromaWhite™ offers several beneficial properties:

• Inhibition of both mushroom and human tyrosinase activity.
• A dose-dependent depigmenting effect on human melanocytes, significantly higher than other agents.
• No cytotoxicity, in contrast to Hydroquinone.
• Photoprotective effects on human epidermal keratinocytes, preventing UV-induced damage.
• Significant skin brightening effects observed in vivo.
• High stability in formulations and good solubility in oils.

Technical Information

ChromaWhite™ is a white to off-white powder with a melting point of 42–44°C and a molecular weight of 446.66 g/mol. It is highly soluble in several common cosmetic solvents, including Ethylhexyl Methoxycinnamate, C12-15 Alkyl Benzoate, and Caprylic/Capric Triglyceride. The recommended dosage for achieving a significant brightening effect is 0.1–0.5%. The product is stable for at least 30 months when stored properly.

Stability

ChromaWhite™ demonstrates reliable stability in O/W emulsions and remains stable across a range of pH levels. Its concentration shows no significant variation over time, even when stored at elevated temperatures. This stability makes it suitable for use in a variety of cosmetic formulations, ensuring consistent performance over time.

Safety

The safety of ChromaWhite™ has been extensively tested both in vitro and in vivo. In vitro tests, including ocular irritation (HET-CAM test), phototoxicity, and cytotoxicity on human keratinocytes and fibroblasts, show no signs of irritation, phototoxicity, or cytotoxicity. Additionally, a bacterial reverse mutation test (Ames test) indicates no mutagenic activity.

In vivo, a Human Repeated Insult Patch Test (HRIPT) conducted on 100 volunteers showed that ChromaWhite™ does not induce skin irritation or sensitization, confirming its safety for cosmetic use.

Efficacy of ChromaWhite™

The efficacy of ChromaWhite™ as a skin brightening agent is one of its most compelling attributes, distinguishing it from other available depigmenting agents. Its effectiveness has been demonstrated through a combination of in vitro (laboratory-based) and in vivo (human trial-based) studies, showing significant inhibition of melanin production and visible skin brightening effects.

In Vitro Efficacy Studies

1. Tyrosinase Inhibition:

   • Tyrosinase is a key enzyme in the synthesis of melanin, making its inhibition a primary target for any effective skin brightening agent. ChromaWhite™ has demonstrated a powerful ability to inhibit the activity of tyrosinase, thereby reducing the production of melanin.
   • At a concentration of 1 mM, ChromaWhite™ inhibited mushroom tyrosinase activity by 37%. Mushroom tyrosinase is often used in preliminary tests due to its similarity to human tyrosinase.
   • More importantly, ChromaWhite™ inhibited human tyrosinase activity by 43% at the same concentration. This level of inhibition is significantly higher than many common skin lighteners, indicating its potent efficacy in reducing melanin synthesis.

2. Reduction of Melanin Production in Melanocytes:

   • Melanocytes are the cells responsible for producing melanin in the skin. In vitro studies involving cultured human melanocytes (cells derived from human skin) have shown that ChromaWhite™ can significantly reduce melanin production.
   • The studies revealed a dose-dependent response, where increasing concentrations of ChromaWhite™ led to a greater reduction in melanin content. This suggests that even at lower concentrations, ChromaWhite™ is effective, making it suitable for various formulations where lower concentrations are preferred.
   • Compared to other depigmenting agents like Kojic Acid, Arbutin, and Magnesium Ascorbyl Phosphate, ChromaWhite™ showed a much higher reduction in melanin production. This highlights its superior efficacy, making it a potent option for combating hyperpigmentation.

3. Non-Cytotoxicity:

   • Unlike other potent skin lightening agents, such as Hydroquinone, which can be cytotoxic (damaging to cells), ChromaWhite™ was found to be non-cytotoxic. This was confirmed by in vitro cytotoxicity tests on human melanocytes, keratinocytes (the predominant cell type in the outer layer of the skin), and fibroblasts (cells that contribute to the formation of connective tissue).
   • The absence of cytotoxic effects is crucial, as it indicates that ChromaWhite™ can inhibit melanin production without damaging the cells that produce it. This safety profile further enhances its appeal as a skin brightening agent.

In Vivo Efficacy Studies

1. Clinical Trials on Human Subjects:

   • The true measure of a skin brightening agent's efficacy is its performance in human trials. ChromaWhite™ was tested on human volunteers in controlled clinical studies to evaluate its ability to reduce hyperpigmentation and brighten the skin.
   • In a 56-day study involving 20 volunteers with visible hyperpigmentation, a formulation containing ChromaWhite™ was applied twice daily. The study results showed a significant reduction in the intensity of pigmentation in the treated areas. The overall skin tone was visibly lighter, and the hyperpigmented spots were less pronounced.

2. Comparative Efficacy:

   • ChromaWhite™ was also tested against other well-known skin lightening agents, including Kojic Acid and Arbutin. In a side-by-side comparison, ChromaWhite™ outperformed these agents in terms of reducing pigmentation.
   • The study found that ChromaWhite™'s efficacy was comparable to that of Hydroquinone, which is often considered the gold standard in skin lightening but with a much better safety profile. This makes ChromaWhite™ an attractive alternative for consumers seeking effective yet safer options.

3. Visual and Quantitative Analysis:

   • The skin-brightening effect of ChromaWhite™ was quantified using a combination of subjective visual assessments and objective measurements, such as colorimetric analysis. Colorimetric analysis measures the changes in skin color to provide an objective assessment of efficacy.
   • The results consistently showed a reduction in melanin content and an overall brightening of the skin tone. Volunteers reported noticeable improvements in skin evenness and a reduction in the appearance of dark spots.

4. Long-Term Efficacy and Stability:

   • Long-term studies indicated that ChromaWhite™ maintains its efficacy over extended periods without causing irritation or sensitization. The brightening effects were sustained, and no rebound hyperpigmentation (a common issue where the skin darkens again after discontinuing the use of some brighteners) was observed.
   • This sustained efficacy, combined with the stability of ChromaWhite™ in various formulations, ensures that products containing this ingredient will continue to perform effectively over time, making it a reliable choice for consumers.

5. Synergistic Effects:

   • ChromaWhite™ also shows potential for synergistic effects when combined with other skin care ingredients. For example, when used alongside sunscreens, its photoprotective properties enhance the overall protection against UV-induced pigmentation.
   • Additionally, it can be incorporated into multi-functional products that target multiple aspects of skin care, such as moisturizing and anti-aging, without compromising its brightening efficacy. This versatility makes it a valuable addition to a wide range of cosmetic products.

Use: for skin care products in any form

How to mix: added in the last step of the formula Avoid using heat.

Utilization rate: 0.1-0.5%

Product characteristics: white to off-white powder

Solubility: can dissolve in oil

Storage: Store in the refrigerator (5-10 degrees). Do not expose to any kind of light. avoid air At least 24 months old

INCI Name : Dimethylmethoxy Chromanyl Palmitate

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