Highly concentrated Bifida (4.5-5.5% dry residue) for very high efficacy of skin barrier preparing. Can see result in only one application (5% in water gel)

Bifida Repair Complex™ (Bifida Ferment Lysate, High Conc)

Highly concentrated Bifida (4.5-5.5% dry residue) for very high efficacy of skin barrier preparing

Example formulation that will offer similar efficacy and texture as Estee Advanced Night Repair

ANR Serum Base 95%

Bifida Repair Complex™ (Bifida Ferment Lysate, High Conc) 5%

Bifida Repair Complex™, a highly concentrated fermentation extract from Bifidobacterium, on enhancing skin barrier function and resisting damage from oxidative stress and inflammation using in vitro models.

Skin barrier enhancement:

- BFL upregulated the expression of genes involved in the skin physical barrier (filaggrin, loricrin, involucrin, transglutaminase 1, aquaporin 3) and antimicrobial barrier (cathelicidin, β-defensin 2) in HaCaT keratinocytes in a dose-dependent manner.
- BFL treatment increased filaggrin protein levels in HaCaT cells.
- These results suggest BFL can promote skin barrier maintenance by inducing expression of barrier-related molecules.

Antioxidant effects:

- BFL exhibited strong free radical scavenging capacity against DPPH, ABTS, hydroxyl, and superoxide radicals in a dose-dependent manner, with highest activity against hydroxyl radicals.
- In H2O2-induced oxidative stress in HaCaT cells, BFL treatment dose-dependently:
    - Increased activities of antioxidant enzymes catalase and glutathione peroxidase
    - Reduced intracellular reactive oxygen species and malondialdehyde levels
- Indicating BFL can protect cells against oxidative damage by boosting antioxidant capacity.

Anti-inflammatory effects:

- In lipopolysaccharide (LPS)-stimulated THP-1 macrophages, BFL significantly inhibited secretion of inflammatory cytokines IL-8 and TNF-α.  
- BFL decreased expression of the inflammatory mediator cyclooxygenase-2 (COX-2).
- Suggesting BFL can modulate the immune response and reduce inflammation.

In summary, the results showed that BFL can reinforce the skin barrier by upregulating barrier genes/proteins and antimicrobial peptides, while also protecting against oxidative stress through antioxidant activity and reducing inflammation by modulating cytokine secretion. The authors conclude BFL strengthens skin defenses against environmental insults like oxidation and inflammation.

(Please see more research detail in the attached file)

Example of products that use Bifida Ferment Lysate in their formulation:

Lancôme Génifique Youth Activating Concentrate Ingredients

Water, Bifida Ferment Lysate, Glycerin, Alcohol Denat., Dimethicone, Hydroxyethylpiperazine Ethane Sulfonic Acid, Sodium Hyaluronate, Phenoxyethanol, PEG-20 Methyl Glucose Sesquistearate, PEG-60 Hydrogenated Castor Oil, Salicyloyl Phytosphingosine, Ammonium Polyacryldimethyltauramide/Ammonium Polyacryloyldimethyl Taurate, Limonene, Xanthan Gum, Caprylyl Glycol, Disodium EDTA, Octyldodecanol, Citric Acid, Citronellol, Parfum/Fragrance.

Estee Lauder Advanced Night Repair Synchronized Recovery Complex Ingredients

Water, Bifida Ferment Lysate, Methylgluceth-20, Peg-75, Butylene Glycol, Bis-Peg-18 Methyl Ether Dimethyl Silane, Arabidopsis Thaliana Extract, Tripeptide-32, Ethylhexyl Methoxycinnamate, Lactobacillus Ferment, Cola Acuminata Extract, Retinyl Palmitate, Pantethine, Caffeine, Glycereth-26, Sodium rna, Squalane, Oleth-3 Phosphate, Oleth-3, Oleth-5, Bisabolol, Choleth-24, Ceteth-24, Hydrogenated Lecithin, Anthemis Nobilis, Sodium Hyaluronate, Tocopheryl Acetate, Lecithin, Xanthan Gum, Tea-Carbomer, Trisodium Edta, Bht, Phenoxyethanol, Methylparaben, Benzyl Alcohol, Green 5, Yellow 5, Red 4.

Usage: Can be used in all types of skin care products

Mixing method: Mix in the water (water-phase). avoid temperature above 40C

Rate of use: 1-7%

Product characteristics: Opaque solution

Solubility: Can dissolve in water

Storage: If you want to keep long-term Keep in the refrigerator at temperature 5-15. ° C avoid light Be at least 2 years old

INCI Name : Water (and) Bifida Ferment Lysate (and) 1,2-Hexanediol (and) Hydroxyacetophenone