UV-VIS Protease Activity Measurement (Substrate: Casein)

Product Code: 125453

Anson and Folin & Ciocalteu method and Horikoshi method

PRINCIPLE

The appearance of peptides is measured as tyrosine equivalent at 660 nm by spectrophotometry.

Service Steps

Step	Procedure	Expected Result
1	Prepare 0.1...	A clear 0.1 N NaOH solution.
2	Prepare 1...	A clear 1 N NaOH solution.
3	Prepare 0.1...	A clear 0.1 M Borax solution.
4	Prepare 0.6%...	A stable, 0.6% casein solution at pH 11.0.
5	Prepare TCA...	A clear TCA mixture solution.
6	Prepare 0.5...	A clear 0.5 M Na2CO3 solution.
7	Prepare 1...	A ready-to-use Folin�s phenol reagent at 1 N concentration.
8	Add 3.0...	Test mixture containing casein and enzyme ready for reaction.
9	Add 3.2...	Reaction stopped with visible precipitate.
10	Centrifuge for...	Clear supernatant obtained for further analysis.
11	Transfer 2...	Test sample supernatant ready for color development.
12	Add exactly...	Slightly alkaline solution ready for Folin�s reagent reaction.
13	Immediately add...	Formation of a blue-colored complex with peptides containing tyrosine.
14	Incubate this...	A stable blue-colored solution proportional to proteolytic activity.
15	Prepare a...	A blank solution for absorbance correction.
16	After incubation,...	Absorbance readings at 660 nm for test and blank samples.
17	Prepare a...	Standard curve generated for protease enzyme activity determination.
18	Use the...	Enzyme activity data recorded in units/mL.
19	Record the...	Absorbance readings of test and blank samples recorded.
20	Subtract the...	Net absorbance calculated for the test sample.
21	Use the...	Final enzyme activity reported in units/mL.

You will receive a report for the Enzyme Activity , Absorbance at 660 nm fields when we provide this service

UV-VIS Protease Activity Measurement (Substrate: Casein)

Anson and Folin & Ciocalteu method and Horikoshi method

PRINCIPLE

The appearance of peptides is measured as tyrosine equivalent at 660 nm by spectrophotometry.

Mechanism	-
Appearance	-
Longevity	-
Strength	-
Storage	-
Shelf Life	-
Allergen(s)	-
Dosage (Range)	-
Recommended Dosage	-
Dosage (Per Day)	-
Recommended Dosage (Per Day)	-
Mix Method	-
Heat Resistance	-
Stable in pH range	-
Solubility	-
Product Types	-
INCI	-

Service Steps

Step	Procedure	Expected Result
1	Prepare 0.1...	A clear 0.1 N NaOH solution.
2	Prepare 1...	A clear 1 N NaOH solution.
3	Prepare 0.1...	A clear 0.1 M Borax solution.
4	Prepare 0.6%...	A stable, 0.6% casein solution at pH 11.0.
5	Prepare TCA...	A clear TCA mixture solution.
6	Prepare 0.5...	A clear 0.5 M Na2CO3 solution.
7	Prepare 1...	A ready-to-use Folin�s phenol reagent at 1 N concentration.
8	Add 3.0...	Test mixture containing casein and enzyme ready for reaction.
9	Add 3.2...	Reaction stopped with visible precipitate.
10	Centrifuge for...	Clear supernatant obtained for further analysis.
11	Transfer 2...	Test sample supernatant ready for color development.
12	Add exactly...	Slightly alkaline solution ready for Folin�s reagent reaction.
13	Immediately add...	Formation of a blue-colored complex with peptides containing tyrosine.
14	Incubate this...	A stable blue-colored solution proportional to proteolytic activity.
15	Prepare a...	A blank solution for absorbance correction.
16	After incubation,...	Absorbance readings at 660 nm for test and blank samples.
17	Prepare a...	Standard curve generated for protease enzyme activity determination.
18	Use the...	Enzyme activity data recorded in units/mL.
19	Record the...	Absorbance readings of test and blank samples recorded.
20	Subtract the...	Net absorbance calculated for the test sample.
21	Use the...	Final enzyme activity reported in units/mL.

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UV-VIS Protease Activity Measurement (Substrate: Casein)

Report will be available after purchasing the service

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