PIPES-2Na, Piperazine-N,N'-bis(2-ethanesulfonic acid) disodium salt
- Product Code: 35441
PIPES-2Na, or Piperazine-N,N'-bis(2-ethanesulfonic acid) disodium salt, is a salt form of the PIPES buffer compound.
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Test Name | Specification |
---|---|
Appearance | White crystalline powder |
Assay (%) | 99-101 |
Solubility (20% aq.) | Clear, colorless solution |
Loss on drying (%) | 3 Max |
pH (1% aq.) | 9.2-10.0 |
Ultraviolet Absorbance (0.1M aq., 260nm) | 0.04 Max |
Ultraviolet Absorbance (0.1M aq., 280nm) | 0.02 Max |
PIPES-2Na, or Piperazine-N,N'-bis(2-ethanesulfonic acid) disodium salt, is a salt form of the PIPES buffer compound.
1. Chemical Structure and Properties:
- PIPES-2Na is the disodium salt of PIPES, formed by neutralizing PIPES with sodium hydroxide.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- PIPES-2Na has a pKa value of around 6.8, similar to the free acid form of PIPES.
2. Applications:
- PIPES-2Na can be used as an alternative to the free acid form of PIPES in various biochemical and molecular biology applications.
- Protein Purification: PIPES-2Na can be used as a buffer component in protein purification techniques, such as ion-exchange chromatography and affinity chromatography.
- Enzyme Assays: PIPES-2Na buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: PIPES-2Na can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: PIPES-2Na may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: PIPES-2Na, like the free acid form, is effective in maintaining a pH range of approximately 6.1-7.5, making it suitable for applications that require a slightly acidic to neutral environment.
- Solubility: PIPES-2Na has excellent solubility in water, allowing for easy preparation of buffer solutions.
- Stability: The sodium salt form of PIPES can be more stable and less prone to degradation compared to the free acid form.
4. Considerations:
- Temperature Dependence: The pH of PIPES-2Na buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: As with any buffer, PIPES-2Na may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, PIPES-2Na is a useful alternative to the free acid form of PIPES, offering similar pH buffering capabilities and potential advantages in terms of solubility and stability.
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PIPES-2Na, or Piperazine-N,N'-bis(2-ethanesulfonic acid) disodium salt, is a salt form of the PIPES buffer compound.
PIPES-2Na, or Piperazine-N,N'-bis(2-ethanesulfonic acid) disodium salt, is a salt form of the PIPES buffer compound.
1. Chemical Structure and Properties:
- PIPES-2Na is the disodium salt of PIPES, formed by neutralizing PIPES with sodium hydroxide.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- PIPES-2Na has a pKa value of around 6.8, similar to the free acid form of PIPES.
2. Applications:
- PIPES-2Na can be used as an alternative to the free acid form of PIPES in various biochemical and molecular biology applications.
- Protein Purification: PIPES-2Na can be used as a buffer component in protein purification techniques, such as ion-exchange chromatography and affinity chromatography.
- Enzyme Assays: PIPES-2Na buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: PIPES-2Na can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: PIPES-2Na may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: PIPES-2Na, like the free acid form, is effective in maintaining a pH range of approximately 6.1-7.5, making it suitable for applications that require a slightly acidic to neutral environment.
- Solubility: PIPES-2Na has excellent solubility in water, allowing for easy preparation of buffer solutions.
- Stability: The sodium salt form of PIPES can be more stable and less prone to degradation compared to the free acid form.
4. Considerations:
- Temperature Dependence: The pH of PIPES-2Na buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: As with any buffer, PIPES-2Na may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, PIPES-2Na is a useful alternative to the free acid form of PIPES, offering similar pH buffering capabilities and potential advantages in terms of solubility and stability.
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