PIPES, Piperazine-N,N'-bis(2-ethanesulfonic acid)
- Product Code: 35440
PIPES, or Piperazine-N,N'-bis(2-ethanesulfonic acid), is a widely used buffer compound in biochemical and molecular biology applications.
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Test Name | Specification |
---|---|
Appearance | White powder |
Assay (%) | 99-101 |
Solubility (5% 1N NaOH) | Clear, colorless solution |
Loss on drying (%) | 0.5 Max |
Ultraviolet Absorbance (20% 1N NaOH) | 0.1 Max |
Heavy metals, ppm | 5 Max |
PIPES, or Piperazine-N,N'-bis(2-ethanesulfonic acid), is a widely used buffer compound in biochemical and molecular biology applications.
1. Chemical Structure and Properties:
- PIPES is a zwitterionic buffer compound containing a piperazine ring with two sulfonic acid groups.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- PIPES has a pKa value of around 6.8, making it useful for maintaining a slightly acidic to neutral pH range.
2. Applications:
- Protein Purification: PIPES is often used as a buffer component in protein purification techniques, such as ion-exchange chromatography and affinity chromatography.
- Enzyme Assays: PIPES buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: PIPES can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: PIPES may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: PIPES is effective in maintaining a pH range of approximately 6.1-7.5, making it suitable for applications that require a slightly acidic to neutral environment.
- Compatibility: PIPES is generally considered a biocompatible buffer and is widely used in biological and biochemical applications.
- Solubility: PIPES has good solubility in water and other polar solvents, allowing for easy preparation of buffer solutions.
4. Considerations:
- Temperature Dependence: The pH of PIPES buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: Like any buffer, PIPES may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, PIPES is a versatile and commonly used buffer compound in a variety of biochemical and molecular biology applications, particularly in the areas of protein purification, enzyme assays, and nucleic acid manipulation.
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PIPES, or Piperazine-N,N'-bis(2-ethanesulfonic acid), is a widely used buffer compound in biochemical and molecular biology applications.
PIPES, or Piperazine-N,N'-bis(2-ethanesulfonic acid), is a widely used buffer compound in biochemical and molecular biology applications.
1. Chemical Structure and Properties:
- PIPES is a zwitterionic buffer compound containing a piperazine ring with two sulfonic acid groups.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- PIPES has a pKa value of around 6.8, making it useful for maintaining a slightly acidic to neutral pH range.
2. Applications:
- Protein Purification: PIPES is often used as a buffer component in protein purification techniques, such as ion-exchange chromatography and affinity chromatography.
- Enzyme Assays: PIPES buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: PIPES can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: PIPES may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: PIPES is effective in maintaining a pH range of approximately 6.1-7.5, making it suitable for applications that require a slightly acidic to neutral environment.
- Compatibility: PIPES is generally considered a biocompatible buffer and is widely used in biological and biochemical applications.
- Solubility: PIPES has good solubility in water and other polar solvents, allowing for easy preparation of buffer solutions.
4. Considerations:
- Temperature Dependence: The pH of PIPES buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: Like any buffer, PIPES may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, PIPES is a versatile and commonly used buffer compound in a variety of biochemical and molecular biology applications, particularly in the areas of protein purification, enzyme assays, and nucleic acid manipulation.
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