CHES, or 2-(Cyclohexylamino)ethanesulfonic acid, is a commonly used buffer compound in biochemical and molecular biology applications.

CHES, or 2-(Cyclohexylamino)ethanesulfonic acid, is a commonly used buffer compound in biochemical and molecular biology applications.

1. Chemical Structure and Properties:

   - CHES is a zwitterionic buffer compound containing a cyclohexylamine group and a sulfonic acid group.
   - It is a white, crystalline powder that is highly soluble in water and other polar solvents.
   - CHES has a pKa value of around 9.5, making it useful for maintaining an alkaline pH range.

2. Applications:

   - Protein Purification: CHES is often used as a buffer component in protein purification techniques, such as ion-exchange chromatography and affinity chromatography.
   - Enzyme Assays: CHES buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
   - Nucleic Acid Manipulation: CHES can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
   - Cell Culture: CHES may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.

3. Advantages:

   - pH Buffering Range: CHES is effective in maintaining a pH range of approximately 8.6-10.0, making it suitable for applications that require an alkaline environment.
   - Compatibility: CHES is generally considered a biocompatible buffer and is widely used in biological and biochemical applications.
   - Solubility: CHES has good solubility in water and other polar solvents, allowing for easy preparation of buffer solutions.

4. Considerations:

   - Temperature Dependence: The pH of CHES buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
   - Potential Interactions: Like any buffer, CHES may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.

Overall, CHES is a versatile buffer compound that is commonly used in a variety of biochemical and molecular biology applications, particularly in the areas of protein purification, enzyme assays, and nucleic acid manipulation.