MOPSO-Na, or Sodium 3-(N-morpholino)-2-hydroxypropanesulfonate, is a salt form of the MOPSO buffer compound

MOPSO-Na, or Sodium 3-(N-morpholino)-2-hydroxypropanesulfonate, is a salt form of the MOPSO buffer compound.

1. Chemical Structure and Properties:

   - MOPSO-Na is the sodium salt of MOPSO, formed by neutralizing MOPSO with sodium hydroxide.
   - It is a white, crystalline powder that is highly soluble in water and other polar solvents.
   - MOPSO-Na has a pKa value of around 7.0, similar to the free acid form of MOPSO.

2. Applications:

   - MOPSO-Na can be used as an alternative to the free acid form of MOPSO in various biochemical and molecular biology applications.
   - Protein Electrophoresis: MOPSO-Na can be used as a buffer component in protein separation techniques, such as SDS-PAGE.
   - Enzyme Assays: MOPSO-Na buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
   - Nucleic Acid Manipulation: MOPSO-Na can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
   - Cell Culture: MOPSO-Na may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.

3. Advantages:

   - pH Buffering Range: MOPSO-Na, like the free acid form, is effective in maintaining a pH range of approximately 6.5-8.0, making it suitable for applications that require a neutral to slightly alkaline environment.
   - Solubility: MOPSO-Na has excellent solubility in water, allowing for easy preparation of buffer solutions.
   - Stability: The sodium salt form of MOPSO can be more stable and less prone to degradation compared to the free acid form.

4. Considerations:

   - Temperature Dependence: The pH of MOPSO-Na buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
   - Potential Interactions: As with any buffer, MOPSO-Na may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.

Overall, MOPSO-Na is a useful alternative to the free acid form of MOPSO, offering similar pH buffering capabilities and potential advantages in terms of solubility and stability.