MOPSO, 3-(N-morpholino)-2-hydroxypropanesulfonic acid
- Product Code: 35434
or 3-(N-morpholino)-2-hydroxypropanesulfonic acid, is a zwitterionic buffer compound that is similar in structure and application to MOPS buffer
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Test Name | Specification |
---|---|
Appearance | White crystal |
Assay (%) | 99-101 |
Solubility (0.5M aq.) | Clear, colorless solution |
Loss on drying (%) | 1 Max |
pH (1% aq.) | 3.9-4.5 |
Ultraviolet absorbance (1M aq., 260nm) | 0.1 Max |
Heavy metals, ppm | 5 Max |
MOPSO, or 3-(N-morpholino)-2-hydroxypropanesulfonic acid, is a zwitterionic buffer compound that is similar in structure and application to MOPS buffer.
1. Chemical Structure and Properties:
- MOPSO is a derivative of MOPS, with an additional hydroxyl group on the propane backbone.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- MOPSO has a pKa value of around 7.0, making it useful for maintaining a neutral pH range.
2. Applications:
- MOPSO is often used as an alternative to MOPS buffer in various biochemical and molecular biology applications.
- Protein Electrophoresis: MOPSO can be used as a buffer component in SDS-PAGE and other protein separation techniques.
- Enzyme Assays: MOPSO buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: MOPSO can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: MOPSO may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: MOPSO is effective in maintaining a pH range of approximately 6.5-8.0, making it suitable for applications that require a neutral to slightly alkaline environment.
- Compatibility: MOPSO is generally considered a biocompatible buffer and is widely used in biological and biochemical applications.
- Solubility: MOPSO has good solubility in water and other polar solvents, allowing for easy preparation of buffer solutions.
4. Considerations:
- Temperature Dependence: The pH of MOPSO buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: As with any buffer, MOPSO may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, MOPSO is a useful alternative to MOPS buffer, offering a similar pH buffering range and applications in biochemical and molecular biology research.
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or 3-(N-morpholino)-2-hydroxypropanesulfonic acid, is a zwitterionic buffer compound that is similar in structure and application to MOPS buffer
MOPSO, or 3-(N-morpholino)-2-hydroxypropanesulfonic acid, is a zwitterionic buffer compound that is similar in structure and application to MOPS buffer.
1. Chemical Structure and Properties:
- MOPSO is a derivative of MOPS, with an additional hydroxyl group on the propane backbone.
- It is a white, crystalline powder that is highly soluble in water and other polar solvents.
- MOPSO has a pKa value of around 7.0, making it useful for maintaining a neutral pH range.
2. Applications:
- MOPSO is often used as an alternative to MOPS buffer in various biochemical and molecular biology applications.
- Protein Electrophoresis: MOPSO can be used as a buffer component in SDS-PAGE and other protein separation techniques.
- Enzyme Assays: MOPSO buffers can help maintain the optimal pH environment for enzymatic reactions and activity measurements.
- Nucleic Acid Manipulation: MOPSO can be used as a buffer in applications involving DNA and RNA, such as gel electrophoresis and enzymatic reactions.
- Cell Culture: MOPSO may be included in cell culture media to help maintain the desired pH range for cell growth and metabolism.
3. Advantages:
- pH Buffering Range: MOPSO is effective in maintaining a pH range of approximately 6.5-8.0, making it suitable for applications that require a neutral to slightly alkaline environment.
- Compatibility: MOPSO is generally considered a biocompatible buffer and is widely used in biological and biochemical applications.
- Solubility: MOPSO has good solubility in water and other polar solvents, allowing for easy preparation of buffer solutions.
4. Considerations:
- Temperature Dependence: The pH of MOPSO buffer solutions can be affected by temperature, so the pH should be carefully monitored and adjusted as needed.
- Potential Interactions: As with any buffer, MOPSO may interact with certain compounds or materials, so its compatibility should be evaluated for specific applications.
Overall, MOPSO is a useful alternative to MOPS buffer, offering a similar pH buffering range and applications in biochemical and molecular biology research.
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