enzymes that can hydrolyze (1→3)-α-glucans, breaking down the biofilm structure and potentially reducing plaque formation

Dental plaque is a biofilm comprising various microorganisms on teeth surfaces. While some microorganisms in the oral biofilm are beneficial, others can overgrow and cause oral diseases. Effective dental plaque control is essential for both oral and systemic health. A key factor in plaque formation is (1→3)-α-glucans, complex sugars produced by bacteria and fungi. These sugars form an insoluble, durable matrix in biofilms. PlaqueCare™ (alpha-1,3-Glucanase) are enzymes that can hydrolyze (1→3)-α-glucans, breaking down the biofilm structure and potentially reducing plaque formation.

Molecular Biology of PlaqueCare™ (alpha-1,3-Glucanase)

Alpha-glucanases belong to two glycoside hydrolase families: GH-71 (fungal origin) and GH-87 (bacterial origin). They are generally monomeric proteins but can also form dimers or tetramers. These enzymes exhibit distinct domain structures: bacterial (1→3)-α-glucanases have a catalytic C-terminal and a supportive N-terminal, while fungal (1→3)-α-glucanases have the reverse arrangement.

Characterization of PlaqueCare™ (alpha-1,3-Glucanase)

Optimal activity for these enzymes varies: bacterial (1→3)-α-glucanases function best at neutral pH (5.5-6.9) and wider pH ranges (4.0-11.0), while fungal (1→3)-α-glucanases prefer acidic conditions (pH 4.5-5.5) and have a narrower stability range (pH 5.0-7.0). Both types of (1→3)-α-glucanases act by hydrolyzing glycosidic bonds in (1→3)-α-glucans, with high specificity, targeting both linear and branched forms.

Use of PlaqueCare™ (alpha-1,3-Glucanase) in oral hygiene products like toothpaste and mouthwashes can disrupt the biofilm matrix by degrading (1→3)-α-glucans, thus reducing plaque formation and preventing oral diseases. Unlike broad-spectrum antimicrobials, (1→3)-α-glucanases specifically target the glycosidic bonds in harmful biofilm sugars without affecting beneficial oral microbiota, offering a promising alternative for oral care formulations.

Usage: Can be used in any oral care product.

How to mix: Dissolve in water phase

Usage rate: For oral dental care products 1-2%

Product appearance: white powder

Solubility: Can be dissolved in water

Storage: For long-term storage Store at cool temperature. Avoid heat and light. Shelf life: 2 years.

Example of product:

DARLIE All Shiny White Supreme Dual Enzyme Formula